Polymersome delivery of siRNA and antisense oligonucleotides
نویسندگان
چکیده
منابع مشابه
Polymersome delivery of siRNA and antisense oligonucleotides.
siRNA and antisense oligonucleotides, AON, have similar size and negative charge and are often packaged for in vitro delivery with cationic lipids or polymers-but exposed positive charge is problematic in vivo. Here we demonstrate loading and functional delivery of RNAi and AON with non-ionic, nano-transforming polymersomes. These degradable carriers are taken up passively by cultured cells aft...
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The potential use of antisense and siRNA oligonucleotides as therapeutic agents has elicited a great deal of interest. However, a major issue for oligonucleotide-based therapeutics involves effective intracellular delivery of the active molecules. In this Survey and Summary, we review recent reports on delivery strategies, including conjugates of oligonucleotides with various ligands, as well a...
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1Medical Research Council Laboratory of Molecular Biology, Cambridge, UK; 2Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK; 3Department of Laboratory Medicine, Karolinska Institute, Hudidnge, Sweden Correspondence: Michael J Gait Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK. E-mail: [email protected]
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Oligonucleotides (ODNs) do not readily get access to their desired site (cytoplasm/nucleus) of action due to their high hydrophility and high molecular weight. Therefore enhancing components (carriers) are needed. Most widely used in vitro are cationic lipids (liposomes). Other systems include peptide conjugates or complexes, polymers and nanoparticles. We used cationic lipids and polymers, wit...
متن کاملTargeted delivery of antisense oligonucleotides by molecular conjugates.
Antisense oligonucleotides efficiently inhibit gene expression in vitro; however, the successful therapeutic application of this technology in vivo will require the development of improved delivery systems. In this report we describe a technique that efficiently delivers antisense oligonucleotides into cells using molecular conjugates. This technique, which was initially developed for the deliv...
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ژورنال
عنوان ژورنال: Journal of Controlled Release
سال: 2009
ISSN: 0168-3659
DOI: 10.1016/j.jconrel.2008.10.020